The recognition of the complexity of flow cytometric analysis has caused many journals and granting organizations to scrutinize flow data. Thus the core flow lab is becoming an important research resource as well as service provider and a prudent investment for academic institutions. The challenge for the academic core facility is to meet both the scientific and technical mission by providing high quality services in a cost-effective and timely manner. Serving 175+ investigators on the Chicago and Evanston Campuses, with 3 sorters and 6 benchtop analyzers with 16+ color capabilities, we have developed a paradigm to balance productivity with quality to minimize the cost per research project, keep overall costs contained, and provide the necessary scientific support. Central to this paradigm is a close working relationship with investigators to define their projects in the early stages of development to make optimal and efficient use of flow cytometry..For more information about this facility, click here.
Imaging & Flow Cytometry Cores
The Electron Probe Instrumentation Center (EPIC) facility offers a wide range of electron microscopy (both transmission and scanning), accessory instrumentation, and expertise to the scientific and engineering community through education, collaboration, and service. The laboratory provides facilities for the preparation and examination of many types of bulk and thin specimens (foils/films), fine particles, and replicas, including biological materials, by transmission and scanning electron microscopy.
Collectively, the Electron Probe Instrumentation Center (EPIC) offers instrumentation, techniques, and expertise for all aspects of microstructure materials. Detailed information about surface morphology, size and shape analysis, local chemistry, crystallography, and texture can be obtained with the scanning electron microscopes (SEM). The SEM facility has five SEMs with digital image acquisition, including four equipped with field emission gun (FEG), EDS, WDS, and EBSD systems. The facility also has a dual beam SEM/FIB and both Ion and Electron Beam Lithography capabilities. The transmission electron microscopes (TEM) allow researchers to probe the crystal structure, defects, local chemistry, electronic structure, and related information at the nanometer or less length scale. The TEM facility currently has four TEMs, including Scanning Transmission Electorn Microscopy (STEM) capabilities. The Hitachi HD2300 STEM is configured with a unique dual EDS system for ultrahigh collection angle EDS, The in-situ S/TEM HT7700 comes with a set of special specimen holders for in-situ experiments. The JEOL JEM2100F FEG TEM/STEM has sub 0.2nm probe capability, equipped with high-angle annular dark field (HAADF) detector, which gives atomic resolution of Z-contrast imaging of STEM. Three of the microscopes are equipped with Liquid Nitrogen cryo stages for biological sample observations. The BioCryo facility of EPIC provides researchers with access to cryo electron microscopy and to an array of electron-probe based imaging and microanalytical methods. Techniques include SE-, TE-, and Z-contrast imaging, electron diffraction, Energy Dispersive X-ray Spectroscopy (EDS), and Electron Energy Loss Spectroscopy (EELS).
Both SEM and TEM facilities are equipped with specialized specimen stages for dynamic studies involving deformation, fracture, current transport, applied electrical and magnetic fields, and temperature variation from -184 ° C to 1000 ° C. The diversity and quality of SEM and TEM instrumentation, along with the numerous analytical accessories, makes EPIC one of the most advanced laboratories in the country. The BioCryo facility offers expertise and support for planning and conducting cryo and conventional electron microscopy studies on biological samples (e.g. suspensions of molecules and particles, cells and tissues), as well as on nanoparticles, polymers, hydrogels, and other materials. Researchers can be trained to become independent users of our instrumentation and techniques. For more information on this facility, click here.
The Biological Imaging Facility (BIF) is a shared-use research and training resource available to all NU researchers. BIF is organized so users can prepare samples, capture and analyze images, and create final presentations in one facility. Training for all instruments is available on a regular basis so users can acquire data quickly and efficiently. We are continuously looking for new ways to enhance existing equipment, acquire new tools, and keep pace with current techniques. For more information about this facility, click here.
The Quantitative Bio-element Imaging Center (QBIC) provides researchers with access to state-of-the-art imaging and quantification instrumentation while supporting its use with an expert technical staff that offers a range of services, including instrument training, sample preparation and analysis, experiment design, and grant proposal assistance. The combination of both extremely high sensitivity elemental analysis and high resolution imaging enables QBIC customers to perform cutting edge experiments with ample staff support. For more information about this facility, click here.
The Center for Translational Imaging (CTI) is the Department of Radiology's MRI research facility that is capable of human and animal (rabbit, rat, mouse) MRI. Most recenty it also provides advancd nuclear imaging capabilities (micro-PET/CT). CTI has the infrastructure and staff to support all types of imaging research studies. For more information about this facility, click here.
The Center for Advanced Microscopy (CAM) at Northwestern offers state-of-the art instrumentation and services for the study of biological processes at the whole animal, tissue, cellular and subcellular levels. The facility's basic services include electron microscopy, super resolution microscopy (SIM & STORM), fluorescent laser scanning and spinning disk microscopy, fluorescent lifetime imaging, fluorescence correlation spectroscopy microscopy, automated high throughput tissue cytometry, laser capture microdissection, mutliphoton imaging, and whole animal bioluminescent and fluorescent imaging. Additionally we provide microinjection equipment, chambers for stable live cell observation, and anesthesia equipment. CAM staff members provide training on numerous different instrument platforms, consultation on experiment design, as well as digital image processing and image analysis. We are a reference site for several companies including TissueGnostics and ISS (developer of the ISS ALBA). CAM is one of three Nikon Imaging Centers in the US, allowing us access and excellent support from Nikon to develop innovative solutions for the cutting edge imaging needs of users. This relationship has allowed our users to push super-resolution technologies to permit multi-color, 3D and live-cell applications. Additionally, we have been working together with Nikon to add confocal laser scanning to a low magnification upright dissection microscope, which is particularly useful to researchers interested in visualizing thick samples (such as epidermal raft cultures) at increased cellular resolution. We are also currently expanding our full service electron microscopy offerings. Recently, we obtained a Leica Freeze Substitution Unit for TEM sample preparation allowing for improved sample quality and streamlined work-flow.
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The Center for Advanced Molecular Imaging (CAMI) provides access to imaging modalities ranging from the nanometer scale to whole animal imaging. These include MRI, nuclear imaging (PET, SPECT, and CT), in vivo bioluminescence and fluorescence imaging, animal housing and prep spaces, and tissue culture. Image analysis services are available, as are software packages (JIM, Amira, Matlab) and a workstation for users to perform their own data analysis. Imaging services can be provided for investigators' own animal models, or animal models can be supplied by the Developmental Therapeutics Core.