Cancer Center (RHLCCC)

Mission

The mission of Northwestern Proteomics is to develop new proteomics technologies, to apply them to timely questions in basic, translational and clinical research, and to educate researchers throughout the Chicagoland area and communicate our research findings to the scientific community at large.

At Northwestern Proteomics, we're committed to providing the best proteomics research on a budget.  We're scientists first, so we will work with you to review each project as it comes in and offer consultation to ensure that everyone's expectations are managed.  That way, the experiment that we perform has the best chance for success.  Proteomics is not a simple technique; so we're both a University Research Center and service-oriented core, handling multiple projects large and small.

Within the Northwestern Proteomics core facility, we offer multiple types of experiments from simple protein identification to protein quantitation (both relative and absolute).  We also perform both traditional bottom-up proteomics, where proteins are digested with an enzyme prior to analysis and intact, top-down proteomics analyses.  The ability to perform top-down proteomics within our core facility distinguishes from many other proteomics cores across the world.

How do you start work with us? 

  • If it’s your first time performing proteomics, we should sit down and chat about your system and the questions that you want to answer.  The diversity of method for proteomics can be overwhelming and we’re happy to help narrow down the best experiment for you.  The first step is to go to our Northwestern Proteomics website (http://proteomics.northwestern.edu) and submit a Collaboration Request.  Someone will be in touch to schedule an in-person or telephone conference.
  • If you’ve worked with us before and already know what you want, head over to our Northwestern Proteomics website and fill out a Sample Submission Form.  Once that’s completed, drop off your sample at either of our dropoff locations Monday through Thursday 8:30-5:00pm and Friday 8:30-12:00pm. 

Drop Off Locations

            Chicago: Olson 8305
            Evanston: Silverman B550

More

The RHLCCC Clinical Research Office (CRO) provides a centralized resource to facilitate the development, conduct, quality assurance monitoring, compliance with regulatory agency requirements, and evaluation of clinical research/trials at the Robert H. Lurie Comprehensive Cancer Center of Northwestern University. As such, the office coordinates the majority of clinical research conducted in medical oncology, malignant hematology, gynecologic-oncology, neuro-oncology, radiation oncology, surgical oncology, and chemoprevention. 

More

The recognition of the complexity of flow cytometric analysis has caused many journals and granting organizations to scrutinize flow data. Thus the core flow lab is becoming an important research resource as well as service provider and a prudent investment for academic institutions. The challenge for the academic core facility is to meet both the scientific and technical mission by providing high quality services in a cost-effective and timely manner. Serving 175+ investigators on the Chicago and Evanston Campuses, with 3 sorters and 6 benchtop analyzers with 16+ color capabilities, we have developed a paradigm to balance productivity with quality to minimize the cost per research project, keep overall costs contained, and provide the necessary scientific support. Central to this paradigm is a close working relationship with investigators to define their projects in the early stages of development to make optimal and efficient use of flow cytometry..For more information about this facility, click here.

More

The  Pathology Core Facility (PCF) of Robert H. Lurie Comprehensive Cancer Center (RHLCCC) at Northwestern University is established in 1997.  PCF is functionally divided into three divisions/units, including core lab, biorepository and clinical trials unit.  Core lab includes all related tasks of routine histology, immunohistochemistry, molecular work, and digital pathology (all aspects of a research-based anatomic pathology laboratory including formalin fixation and paraffin embedding, automated and manual immunohistochemistry, tissue microarray design and construction, and automated whole slide scanning for digital pathology applications, including advanced image analysis capabilities). Biorepository division includes tissue and biospecimen procurement (a workflow that complements clinical patient care, the consent procedures which include statement of basic, translational and clinical research and PDX studies for potential therapies), detailed sample annotation (pre-analytic variables such as cold post-surgery time, frozen section evaluation, specimen tracking, and a quality management program); and well-controlled biospecimens and procedures (ensuring that clinically meaningful and reproducible data emerge from investigation). The clinical trials unit (CTU) provides service of institution, regional and national network and prepares trial cases of fast turnaround time and high quality trial materials and patients’ satisfaction. In conjunction with the Cancer Center’s Clinical Trials Office, the clinical trials unit participates in both industry based clinical trials and investigator initiated clinical trials. 

More

The Keck Biophysics Facility provides researchers with 24-hour access to a collection of advanced instruments for biophysical and biochemical characterization of macromolecules and their interactions. Training, technical expertise and assistance are provided by the facility personnel. Staff service on selected instruments is available. For more information about this facility, click here.

More

The mission of the Mary Beth Donnelley Clinical Pharmacology Core Facility is to provide state-of-the-art clinical pharmacologic support to Northwestern University investigators as an integral part of bench-to-bedside drug development.  The Clinical Pharmacology Core provides pharmacokinetic support for preclinical and clinical studies of cancer chemotherapeutic agents, analgesics, and other small molecules.  Expertise includes optimizing the design, conduct, analysis, interpretation, and reportage of pharmacokinetic studies.  Small molecule concentrations in plasma and other body fluids are measured using an Agilent HPLC system linked to an Applied Biosystems API 3000 triple quadrupole mass spectrometer.  Drug concentration histories are fitted to various compartmental pharmacokinetic models using commercially available and specialized software. For more information about this facility, click here.

More

The Center for Advanced Microscopy (CAM) at Northwestern offers state-of-the art instrumentation and services for the study of biological processes at the whole animal, tissue, cellular and subcellular levels. The facility's basic services include electron microscopy, super resolution microscopy (SIM & STORM), fluorescent laser scanning and spinning disk microscopy, fluorescent lifetime imaging, fluorescence correlation spectroscopy microscopy, automated high throughput tissue cytometry, laser capture microdissection, mutliphoton imaging, and whole animal bioluminescent and fluorescent imaging. Additionally we provide microinjection equipment, chambers for stable live cell observation, and anesthesia equipment. CAM staff members provide training on numerous different instrument platforms, consultation on experiment design, as well as digital image processing and image analysis. We are a reference site for several companies including TissueGnostics and ISS (developer of the ISS ALBA). CAM is one of three Nikon Imaging Centers in the US, allowing us access and excellent support from Nikon to develop innovative solutions for the cutting edge imaging needs of users. This relationship has allowed our users to push super-resolution technologies to permit multi-color, 3D and live-cell applications. Additionally, we have been working together with Nikon to add confocal laser scanning to a low magnification upright dissection microscope, which is particularly useful to researchers interested in visualizing thick samples (such as epidermal raft cultures) at increased cellular resolution. We are also currently expanding our full service electron microscopy offerings. Recently, we obtained a Leica Freeze Substitution Unit for TEM sample preparation allowing for improved sample quality and streamlined work-flow.

Please feel free to contact us with questions.

For the Research Newsletter featuring CAM, please click here.

More

The Outcomes Measurement and Survey Core (OMSC) serves the needs of the cancer researchers within the Robert H. Lurie Comprehensive Cancer Center. The mission of this core facility is to provide consultation and support for research that involves collecting, analyzing or interpreting self-report data in culturally diverse populations. The OMSC is housed within the Department of Medical Social Sciences, which also provides similar services to any member of the Northwestern community. For more information about this facility click here.

More

The Mouse Histology and Phenotyping Laboratory (MHPL) is designed to assist investigators with their research histology needs, as well as gross and histological characterization of genetically modified murine models. Studies can be performed on individual organs or involve a systemic overview of all major organ systems. Pathologist consultation will allow the development of strategies to elucidate the phenotype and gain mechanistic insight regarding the biologic actions of the targeted molecule. For more information about this facility, click here.

MHPL Homepage

More